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psip1 pwwp 1 135 domain 40744 constructs  (Addgene inc)


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    Structured Review

    Addgene inc psip1 pwwp 1 135 domain 40744 constructs
    Th-T based aggregation kinetics of 25 μM αSyn 1–103 in the presence of 25 μM representative proteins from each group: LB components (PIN1, LH1.5_GD), known interactors (BSA, lysozyme, SIRT6, and Fyn-SH3 domain), and uncharacterized binders (RNase, TEV protease, GST, <t>and</t> <t>PSIP1-PWWP</t> domain). Controls include αSyn 1–103 alone (red) and individual proteins alone (yellow). Data represent the mean of three individual triplicates ( n = 3) and the error bar shows ±SD. Representative T₅₀ values, derived from one-phase exponential association kinetics, for αSyn 1–103 control and its 1:1 mixtures with cellular proteins are presented as a comparative bar graph. The dashed line indicates the T₅₀ value for the αSyn 1–103 control.
    Psip1 Pwwp 1 135 Domain 40744 Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/psip1+pwwp/pmc12877186-343-7-15?v=Addgene+inc
    Average 94 stars, based on 1 article reviews
    psip1 pwwp 1 135 domain 40744 constructs - by Bioz Stars, 2026-08
    94/100 stars

    Images

    1) Product Images from "Parkinson’s disease-specific α-Synuclein variants potentially drive Lewy body formation by engaging in promiscuous and non-functional interactions"

    Article Title: Parkinson’s disease-specific α-Synuclein variants potentially drive Lewy body formation by engaging in promiscuous and non-functional interactions

    Journal: Communications Biology

    doi: 10.1038/s42003-025-09395-9

    Th-T based aggregation kinetics of 25 μM αSyn 1–103 in the presence of 25 μM representative proteins from each group: LB components (PIN1, LH1.5_GD), known interactors (BSA, lysozyme, SIRT6, and Fyn-SH3 domain), and uncharacterized binders (RNase, TEV protease, GST, and PSIP1-PWWP domain). Controls include αSyn 1–103 alone (red) and individual proteins alone (yellow). Data represent the mean of three individual triplicates ( n = 3) and the error bar shows ±SD. Representative T₅₀ values, derived from one-phase exponential association kinetics, for αSyn 1–103 control and its 1:1 mixtures with cellular proteins are presented as a comparative bar graph. The dashed line indicates the T₅₀ value for the αSyn 1–103 control.
    Figure Legend Snippet: Th-T based aggregation kinetics of 25 μM αSyn 1–103 in the presence of 25 μM representative proteins from each group: LB components (PIN1, LH1.5_GD), known interactors (BSA, lysozyme, SIRT6, and Fyn-SH3 domain), and uncharacterized binders (RNase, TEV protease, GST, and PSIP1-PWWP domain). Controls include αSyn 1–103 alone (red) and individual proteins alone (yellow). Data represent the mean of three individual triplicates ( n = 3) and the error bar shows ±SD. Representative T₅₀ values, derived from one-phase exponential association kinetics, for αSyn 1–103 control and its 1:1 mixtures with cellular proteins are presented as a comparative bar graph. The dashed line indicates the T₅₀ value for the αSyn 1–103 control.

    Techniques Used: Derivative Assay, Control



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    psip1 pwwp 1 135 domain 40744 constructs  (Addgene inc)
    94
    Addgene inc psip1 pwwp 1 135 domain 40744 constructs
    Th-T based aggregation kinetics of 25 μM αSyn 1–103 in the presence of 25 μM representative proteins from each group: LB components (PIN1, LH1.5_GD), known interactors (BSA, lysozyme, SIRT6, and Fyn-SH3 domain), and uncharacterized binders (RNase, TEV protease, GST, <t>and</t> <t>PSIP1-PWWP</t> domain). Controls include αSyn 1–103 alone (red) and individual proteins alone (yellow). Data represent the mean of three individual triplicates ( n = 3) and the error bar shows ±SD. Representative T₅₀ values, derived from one-phase exponential association kinetics, for αSyn 1–103 control and its 1:1 mixtures with cellular proteins are presented as a comparative bar graph. The dashed line indicates the T₅₀ value for the αSyn 1–103 control.
    Psip1 Pwwp 1 135 Domain 40744 Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/psip1+pwwp/pmc12877186-343-7-15?v=Addgene+inc
    Average 94 stars, based on 1 article reviews
    psip1 pwwp 1 135 domain 40744 constructs - by Bioz Stars, 2026-08
    94/100 stars
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    psip1 pwwp  (Addgene inc)
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    Th-T based aggregation kinetics of 25 μM αSyn 1–103 in the presence of 25 μM representative proteins from each group: LB components (PIN1, LH1.5_GD), known interactors (BSA, lysozyme, SIRT6, and Fyn-SH3 domain), and uncharacterized binders (RNase, TEV protease, GST, <t>and</t> <t>PSIP1-PWWP</t> domain). Controls include αSyn 1–103 alone (red) and individual proteins alone (yellow). Data represent the mean of three individual triplicates ( n = 3) and the error bar shows ±SD. Representative T₅₀ values, derived from one-phase exponential association kinetics, for αSyn 1–103 control and its 1:1 mixtures with cellular proteins are presented as a comparative bar graph. The dashed line indicates the T₅₀ value for the αSyn 1–103 control.
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    recombinant dna reagent gst-psip1-pwwp (residues 3–100) for bacteria expression  (NovoPro Biosciences Inc)
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    Th-T based aggregation kinetics of 25 μM αSyn 1–103 in the presence of 25 μM representative proteins from each group: LB components (PIN1, LH1.5_GD), known interactors (BSA, lysozyme, SIRT6, and Fyn-SH3 domain), and uncharacterized binders (RNase, TEV protease, GST, <t>and</t> <t>PSIP1-PWWP</t> domain). Controls include αSyn 1–103 alone (red) and individual proteins alone (yellow). Data represent the mean of three individual triplicates ( n = 3) and the error bar shows ±SD. Representative T₅₀ values, derived from one-phase exponential association kinetics, for αSyn 1–103 control and its 1:1 mixtures with cellular proteins are presented as a comparative bar graph. The dashed line indicates the T₅₀ value for the αSyn 1–103 control.
    Recombinant Dna Reagent Gst Psip1 Pwwp (Residues 3–100) For Bacteria Expression, supplied by NovoPro Biosciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Th-T based aggregation kinetics of 25 μM αSyn 1–103 in the presence of 25 μM representative proteins from each group: LB components (PIN1, LH1.5_GD), known interactors (BSA, lysozyme, SIRT6, and Fyn-SH3 domain), and uncharacterized binders (RNase, TEV protease, GST, and PSIP1-PWWP domain). Controls include αSyn 1–103 alone (red) and individual proteins alone (yellow). Data represent the mean of three individual triplicates ( n = 3) and the error bar shows ±SD. Representative T₅₀ values, derived from one-phase exponential association kinetics, for αSyn 1–103 control and its 1:1 mixtures with cellular proteins are presented as a comparative bar graph. The dashed line indicates the T₅₀ value for the αSyn 1–103 control.

    Journal: Communications Biology

    Article Title: Parkinson’s disease-specific α-Synuclein variants potentially drive Lewy body formation by engaging in promiscuous and non-functional interactions

    doi: 10.1038/s42003-025-09395-9

    Figure Lengend Snippet: Th-T based aggregation kinetics of 25 μM αSyn 1–103 in the presence of 25 μM representative proteins from each group: LB components (PIN1, LH1.5_GD), known interactors (BSA, lysozyme, SIRT6, and Fyn-SH3 domain), and uncharacterized binders (RNase, TEV protease, GST, and PSIP1-PWWP domain). Controls include αSyn 1–103 alone (red) and individual proteins alone (yellow). Data represent the mean of three individual triplicates ( n = 3) and the error bar shows ±SD. Representative T₅₀ values, derived from one-phase exponential association kinetics, for αSyn 1–103 control and its 1:1 mixtures with cellular proteins are presented as a comparative bar graph. The dashed line indicates the T₅₀ value for the αSyn 1–103 control.

    Article Snippet: Pin1 FL (#40773), Tau FL (#16316), and PSIP1-PWWP (1-135) domain (#40744) constructs were obtained from Addgene. (His) 6 -tagged proteins (Pin1 and PSIP1-PWWP domain) were expressed in E. coli BL21(DE3) using 0.5 mM IPTG induction at 37°C for 6 h and purified initially using Ni 2+ -affinity chromatography (HiTrap IMAC FF, Cytiva).

    Techniques: Derivative Assay, Control